Journal: Scientific Reports

Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury

doi: 10.1038/s41598-025-34083-3

Figure Lengend Snippet: UMI-77 alleviates HK-2 cells apoptosis after hypoxia reoxygenation. ( A ) Representative images and ( B ) quantification of flow cytometric analysis of apoptotic HK-2 cells (Q2 + Q3). ( C ) Cell viability anylasis by CCK-8 detection. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The human proximal tubular epithelial cell line HK-2 cells (American Type Culture Collection) was obtained from Shanghai Jiaotong University Affiliated Hospital.

Techniques: CCK-8 Assay

Journal: Scientific Reports

Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury

doi: 10.1038/s41598-025-34083-3

Figure Lengend Snippet: UMI-77 induces mitophagy in H/R treated HK-2 cells. ( A , B ) Co-localization of TOM20 (green) and LAMP1 (red) in different groups HK-2cells was detected using double fluorescence staining, followed by analysis with metamorph. Nuclei were stained with DAPI. Boxed areas are magnified and shown in bottom panel. Scale bars = 10 μm, 1800× magnification). ( C ) Representative electron micrograph of mitophagosome in HK-2cells with or without UMI-77 treatment in vitro. Scale bar = 1 μm (30000× magnification) in up panel and 500 nm (60000× magnification) in bottom panel.

Article Snippet: The human proximal tubular epithelial cell line HK-2 cells (American Type Culture Collection) was obtained from Shanghai Jiaotong University Affiliated Hospital.

Techniques: Fluorescence, Staining, In Vitro

Journal: Scientific Reports

Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury

doi: 10.1038/s41598-025-34083-3

Figure Lengend Snippet: Mdivi-1 pretreatment partially counteracts the protective effect of UMI-77 on H/R treated HK-2 cells. ( A ) Representative images and ( B ) quantification of flow cytometric analysis of the effect of UMI-77 on apoptosis of Mdivi-1 pretreated HK-2 cells. ( C ) The intracellular MitoSOX fluorescence intensity was observed under confocal microscope, and the intracellular mtROS level was detected. Scale bars = 20 μm, 600× magnification. ( D ) JC-1 fluorescent probe was used to label each group of HK-2 cells. The fluorescence expression of JC-1 polymer (red) and JC-1 monomer (green) was observed under confocal microscope to detect the MMP of HK-2 cells. Scale bars = 20 μm, 600× magnification. ( E ) Representative immunoblotting images of p62, TOM20, and COXIV.

Article Snippet: The human proximal tubular epithelial cell line HK-2 cells (American Type Culture Collection) was obtained from Shanghai Jiaotong University Affiliated Hospital.

Techniques: Fluorescence, Microscopy, Expressing, Polymer, Western Blot

Journal: Scientific Reports

Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury

doi: 10.1038/s41598-025-34083-3

Figure Lengend Snippet: Localization detection of MCL-1 expression in UMI-77 treated HK-2 cells and validation of MCL-1 knockdown efficiency. ( A ) Representative images of dual-labeled immunofluorescence staining of TOM20 and MCL-1 in UMI-77 treated HK-2 cells. Scale bars = 10 μm, 1800× magnification. ( B ) Western blot assay of MCL-1 in MCL-1 knockdown HK-2 cells. ( C ) qRT-PCR analyses of MCL-1 mRNA expression in MCL-1 knockdown HK-2 cells.

Article Snippet: The human proximal tubular epithelial cell line HK-2 cells (American Type Culture Collection) was obtained from Shanghai Jiaotong University Affiliated Hospital.

Techniques: Expressing, Biomarker Discovery, Knockdown, Labeling, Immunofluorescence, Staining, Western Blot, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury

doi: 10.1038/s41598-025-34083-3

Figure Lengend Snippet: Knockdown of MCL-1 partially counteracts the protective effect of UMI-77 on H/R treated HK-2 cells. ( A ) Representative images and ( B ) quantification of flow cytometric analysis of the effect of UMI-77 on apoptosis of MCL-1 knockdown HK-2 cells. ( C ) The intracellular MitoSOX fluorescence intensity was observed under confocal microscope, and the intracellular mtROS level was detected. Scale bars = 20 μm, 600× magnification. ( D ) JC-1 fluorescent probe was used to label each group of HK-2 cells. The fluorescence expression of JC-1 polymer (red) and JC-1 monomer (green) was observed under confocal microscope to detect the MMP of MCL-1 knockdown HK-2 cells. Scale bars = 20 μm, 600× magnification. (E) Representative immunoblotting images of TOM20, and COXIV.

Article Snippet: The human proximal tubular epithelial cell line HK-2 cells (American Type Culture Collection) was obtained from Shanghai Jiaotong University Affiliated Hospital.

Techniques: Knockdown, Fluorescence, Microscopy, Expressing, Polymer, Western Blot